Cover of Nucleic Acids Research, December 2001
2001 December 1 issue of
Nucleic Acids Research
Copyright 2001
Oxford University Press.

Flippers, Flappers and Flip-Flops in DNA Binding

Abstract of a talk by Thomas D. Schneider
part of a circuit board in black and white lines

tiny gumball machine The mathematics of information theory provides powerful tools for cracking problems in molecular biology. For example, the packing of spheres in high dimensional spaces is directly related to molecular states. More details about this topic can be found at: http://schneider.ncifcrf.gov/. In this talk I will show how we used information theory to understand two kinds of genetic control system.

Genetic control is often done in a simple way: a protein binds to a spot near the start of a gene and physically prevents the gene from being used (that is, it blocks the DNA from being transcribed into RNA). The amount of pattern in the DNA can be measured in bits of information, and this reveals that the protein shape molds to the surface of the DNA during evolution. DNA has major (ie wide) and minor (ie narrow) grooves and proteins can get more information from the major groove (2 bits) than they can from the minor groove (1 bit). This can be seen clearly using information theory.

In the first genetic system, a virus called P1 replicates its DNA in the intestinal bacterium Escherichia coli by using a control protein called RepA. Surprisingly, RepA violates the idea given above. When a theory appears to fail it can teach us new things. In this case RepA probably is flipping a base out of the DNA (swivelling half of a DNA ladder rung out of the ladder) in order to start the opening of DNA for DNA replication. Strong experimental evidence was found to support this hypothesis.

In the second genetic system, we discovered that the Fis protein (which controls all kinds of stuff in E. coli frequently uses pairs of sites 7 or 11 base pairs apart on DNA. Two overlapping Fis sites separated by 11 base pairs are found in the E. coli origin of chromosomal replication (the place where replication begins). We found that only one of the two overlapping Fis sites is bound by Fis at a time, so the structure is a molecular flip-flop. Since the two sites are precisely positioned between two DnaA sites, and these determine the orientation of the DnaB helicase, we suggest that the flip-flop directs alternative firing of replication complexes in opposite directions. Since they can implement Boolean logic, molecular flipflops could be used to build molecular computers. Both of these discoveries were made only because of the clarity that information theory provides.

References:

color bar Small icon for Theory of Molecular Machines: physics,
chemistry, biology, molecular biology, evolutionary theory,
genetic engineering, sequence logos, information theory,
electrical engineering, thermodynamics, statistical
mechanics, hypersphere packing, gumball machines, Maxwell's
Daemon, limits of computers


Schneider Lab

origin: 2001 2001 Dec 9
updated: 2001 2001 Dec 12
color bar
U.S. Department of Health and Human Services  |  National Institutes of Health  |  National Cancer Institute  |  USA.gov  | 
Policies  |  Viewing Files  |  Accessibility  |  FOIA