Delila Program: titer

titer program

Documentation for the titer program is below, with links to related programs in the "see also" section.

{const   version = 2.29;  (* of titer.p 1993 Jan 27}

(* begin module describe.titer *)
(*
name
      titer: analyse titertek optical density data

files
      titer(plates: in, result: out, verbose: out, output: out)

synopsis
      plates: output of the tk program; containing a header line,
         and a series of plates that describe the names of wells,
         their optical densities at various times and od620 data.
      result: a tabulation of the beta-galactosidase values.
      verbose: more detail on how the calculations were done.
      output: messages to the user.

description
      take data from titertek plates and do the analysis:
        get sample names from id plate, find duplicates
        read in volume values
        read in od620 values
        read in od414 values for each time point
                calculate best slope from all time points
                calculate activity for each sample
        report beta-galactosidase data
           st.dev. is the standard deviation for the samples
           % dev.  is 100*st.dev./activity.  If this is larger than 10%
                   for 4 samples, we usually redo the measurement.

examples
        titer.plates  example input file
        titer.result  example output file
        titer.verbose  example output file

see also
        beta-galactosidase assay protocol
        tk program (in advanced IBM basic)
        tkod.p program

author
        Gary Stormo

*)
(* end module describe.titer *)
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